How to Make Buttermilk at Home in 5 Minutes

Never stall your baking again with this simple, two-ingredient kitchen hack that yields perfectly tangy buttermilk in minutes. This homemade substitute provides the essential acidity needed to activate leavening agents for the fluffiest pancakes and most tender biscuits you've ever tasted.

By Stephanie Robertson, Recipe Editor

March 12, 2026

Step 1. Pour one tablespoon of fresh lemon juice or distilled white vinegar into a liquid measuring cup. Step 2. Add enough milk to the measuring cup until the liquid reaches the 1-cup (240ml) mark. Step 3. Give the mixture a quick stir with a spoon to ensure the acid is fully incorporated. Step 4. Let the mixture sit undisturbed at room temperature for 5 to 10 minutes. Step 5. Once the milk has slightly thickened and developed small curdled bits, stir it once more and use it immediately in your favorite recipe.

To put the method into practice, try buttermilk pancakes. To put the method into practice, try buttermilk fried chicken.

Key Takeaways

✓ Use room temperature milk for the fastest curdling results.
✓ While lemon juice adds a bright flavor, white vinegar is the most neutral option for savory bakes.
✓ Avoid using ultra-pasteurized milk if possible, as it may not curdle as effectively.
✓ If you need a thicker consistency, swap the milk for heavy cream or half-and-half.

Frequently Asked Questions

Can I use low-fat or skim milk?

Yes, you can use 1% or 2% milk, but the resulting buttermilk will be thinner and less rich than a whole milk version.

How long does homemade buttermilk last?

It is best used immediately for the most active leavening, but you can store it in an airtight container in the fridge for up to 3 days.

Can I make a vegan buttermilk substitute?

Absolutely. Use soy milk or almond milk with the same amount of lemon juice or vinegar. Soy milk typically curdles the best of the plant-based options.

Does this work for drinking?

This substitute is specifically designed for baking and marinades. It will not have the exact same buttery flavor or thick texture as traditional churned buttermilk for drinking.